Microscopes
Microscopy is widely used across all fields of natural sciences. See examples of applications.
Microscopy is widely used across all fields of natural sciences. See examples of applications.
Microscopy is very widely used across all fields of natural sciences. Since the development of the first very simple microscopes in the 17th century, a wide range of new technologies and methods have been born, the diffraction limit has been broken and it is now possible to image samples the size of an entire organism (zebrafish, octopus). The resolution in space and time continues to improve and modern microscopes produce a vast amount of information that pushes the boundaries of modern science.
New super-resolution modules and the patented B-TIRF reveal even the smallest details, such as the dynamics of viral infection or the ultrastructure of chromatin or organelles.
Confocal microscopy enables fast and high-quality imaging at high resolution. The acquired data in the form of optical sections must be reconstructed and subsequently analyzed. A 3D colocalization study is a reliable method that reveals structural relationships between imaged objects.
Deconvolution in microscopy is a widespread method for image data processing. The microscope introduces a certain defect (PSF - convolution) into the acquired data during imaging. Deconvolution algorithms work with its parameters and correct the data to obtain a higher resolution image that better represents the object under study.
The anatomy of the neural network allows the brain to function properly. Confocal microscopy and subsequent analysis of the imaged data is a way to understand the anatomy and physiology of neural circuits and relationships within neural networks.
Thousands of in situ hybridization experiments need to be analyzed quickly and qualitatively using SD confocal microscopy (spinning disc confocal microscopy).
Visualization of cells and cellular structures in three-dimensional form brings with it greater demands on image processing than in the case of classical 2D microscopy. Confocal microscopes allow to image and measure objects in a non-destructive way at very high resolution, and by scanning tens to hundreds of optical sections, it is possible to reconstruct the resulting image of a single region in 3D dimensions using a suitable analysis program.
Organoids, or complex structures of cell cultures, are suitable candidates for in vivo observation of biological processes with a confocal microscope.
Optogenetics- the control of cells in tissue by light, is a popular method for monitoring processes in nervous tissue.
FRAP (fluorescence recovery after photobleaching): a fluorescence imaging method for studying dynamic processes in cell and molecular biology.
Live cell imaging: a progressive method in cell biology focused on the study of living cell structures and their interactions with tissues or other materials.
Light sheet microscopy has become very popular for imaging living cells and organisms due to its imaging speed and low photo-toxicity. There are a large number of microscopes that are based on similar technology. So far, commercial solutions implement only some of these capabilities.
The Andor Dragonfly spinning disk confocal microscope is perfect for plant biologists. The multi-modal concept allows imaging of a wide range of samples, from miniature plant cell nuclei to entire root sections. It is also possible to image large sample volumes or multiple layers very quickly in time lapse experiments.
Confocal microscopy is based on the suppression of the signal originating outside the focal plane. The types of confocal microscopes are CLSM and SDCM.