SMLM (Single Molecule Localization Microscopy), or single molecule localization microscopy, is one of the techniques for obtaining super-resolutionimages by observing and localizing single fluorescent molecules with a precision significantly greater than the conventional microscope resolution. The accuracy of localizing individual molecules is affected by the magnitude of the image noise, which decreases with the total number of photons in the measured PSF(Point Spread Function). For example, when 106 photons are detected, the accuracy of molecule localization approaches 1 nm. The principle of SMLM is identical for three methods that have been discovered independently - PALM(PhotoActivation Localization Microscopy), FPALM (Fluorescence PhotoActivation Localization Microscopy) and STORM(Stochastic Optical Reconstruction Microscopy), and which differ from each other in the nature of the photoactivatable fluorescent molecules used and their corresponding mechanisms for generating fluorescent molecules.