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RMS1000 confocal Raman microscope

The new RMS1000 is a versatile, high-end confocal Raman microscope from Edinburgh Instruments that has been designed to suit almost any modern, state-of-the-art Raman spectroscopy application. However, the RMS1000 also enables other methods of investigating biochemical and biophysical systems, such as time-resolved fluorescence (TRFM), fluorescence lifetime imaging (FLIM) and photoluminescence microscopy.

Key Features

  • 5 integrated lasers + possibility to add additional external lasers
  • Flexibility - upright and inverted microscope options
  • Diffraction grating carousel with 5 positions
  • Up to two spectrographs can be integrated
  • Truly confocal - more than 10 pinhole positions available from 25 µm to 100 µm
  • Motorised slitsize adjustable from 20 µm to 100 µm as standard, up to a maximum of 500 µm
  • Motorised carousel for 6 Raman filters
  • Filter wheel for laser intensity adjustment from 0.01% to 100%
  • Up to four simultaneous detectors
  • Internal standards and automatic calibration (Neon, Silicon, White light standards)
  • User-friendly Ramacle® software for complete system control, data acquisition, display and analysis
  • Additional methods available - photoluminescence microscopy, time-resolved fluorescence, FLIM
  • System supplied with laser blocking when the instrument cover is opened - safety class Class I, or without laser cover - safety class 3B

Aplikační oblasti:

Biological Sciences | Pharmacy | Chemistry, Analysis and Control of Chemicals | Polymers | Nanomaterials | Cosmetics | Semiconductors | Art & Museum - Material Analysis | Forensic Analysis | Geology

Miroslav Kořínek
Expert advisor

RNDr. Miroslav Kořínek Ph.D.

+420 720 994 252

korinek@optixs.cz

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OptiXs care

  • We will expertly consult your planned application
  • Our team is able to integrate the product into a larger system
  • We ensure fast delivery of spare parts and local service
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Product description

It is a true confocal system with an adjustable motorized slit and multi-position adjustable pinhole for higher image resolution, better fluorescence suppression and overall application optimization. It is based on the previous model, the RM5 confocal Raman microscope.

The RMS1000 includes up to 5 integrated, computer controlled narrowband lasers with the ability to connect severaladditional external lasers. Pulsed lasers, either integrated directly into the system or connected externally, can also be used for fluorescence lifetime measurements (FLIM) and advanced Raman measurements.

The system includes up to two spectrographs, each with a 5 position carousel for diffraction gratings for the highest possible spectral resolution (from < 0.1 cm-1, coverage 5 cm-1 - 30,000 cm-1), suitable gratings are supplied with each laser.

Up to 4 detectors can be installed - high efficiency TE-cooled CCD, EMCCD, InGaAs and others.

Of course, integrated internal standards and auto-calibration procedures are provided to ensure the highest quality of acquired data, as well as the powerful Ramacle software package for complete system control, data acquisition and subsequent analysis. A major advantage for RMS1000 users is access to the KnowItAll Raman Identification Pro spectral library, which is available for material identification and advanced analysis.

Data acquisition methods such as single measurements, multiple and accumulated scans, kinetic scans and map generation (1D, 2D and 3D mapping depending on accessories) are implemented with intuitive and user-friendly wizards.

Data processing: smoothing, basic correction, fluorescence background correction, cosmic ray removal, spectral range clipping, spectral normalization, basic arithmetic functions

Key features of Ramacle® software:

  • Selection of laser and scattering optical paths
  • Selection of excitation wavelength, diffraction gratings and exposure time
  • Visualization of sample and laser focus
  • Programmable attenuator and shutter
  • Acquisition of single, accumulated and kinetic spectra
  • Spectral correction
  • Selection of internal calibration standards and automated calibration correction
  • Data operations such as arithmetic, scaling, normalization and baseline subtraction
  • Cosmic ray removal, clipping, smoothing
  • Automated laser beam positioning
  • ASCII/CSV data import/export function
  • Presentation and publication options
  • Mapping functions - map setup, data collection and analysis
  • Fully motorized XYZ table - joystick and software control
  • Polarizer and analyzer selection and control (for advanced polarization analysis of Raman spectra)
  • Selection of detectors, filters, external camera
  • Optional add-on module for chemometric analysis and KnowItAllTM spectrum library package

Video

Application

Parameters

Spectral resolution from < 0.1 cm-1 (depending on choice of grating, laser and CCD detector)
Spatial resolution XY, Z 0.25 μm, < 1 μm (depending on the laser and microscope objective used)
Spectral range 5 cm-1 - 30,000 cm-1
Wavelength range 200 nm - 2,200 nm
Lasers Typically 532 nm, 638 nm, 785 nm, wavelengths available from UV to NIR
CCD detector 1650 x 200 pixels, TE-cooled -60°C (standard) 2000 x 256 pixels, TE-cooled -60°C (improved sensitivity/spectral range)
EMCCD detector InGaAs detector 1600 x 200 pixels, TE-cooling -100°C (fast response time) 1024 pixels, TE-cooling -90°C up to 2,200 nm

Dokumenty

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Are you interested in product? Send us your requirements via the enquiry form or contact an expert consultant directly. We will be happy to answer your questions and propose a solution according to your needs.

Expert advisor

Miroslav Kořínek

RNDr. Miroslav Kořínek Ph.D.

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