Client's assignment and requirements:
- Optical confocal microscope for nanostructure characterization and quantum technology research
- Sufficient number of excitation sources covering a wide part of the visible spectrum
- Ability to work with photosensitive samples
- Combination of several imaging modalities - confocal imaging for fast scanning, widefield epifluorescence for detection of weak signals and transmitted light mode for transparent samples
- Highly compact microscope
- Vibration cancellation system
- Real-time 3D data display capability during acquisition with full capability to not only monitor the experiment, but also to actively input into the process, including termination
Our solution:
Andor 's BC43 multi-modal benchtop confocal microscope with a compact footprint, built-in anti-vibration platform and a tight, opaque housing.
The microscope offers several imaging modes for different sample types and their combination with each other:
- High-speed confocal imaging mode based on a dual rotating disk with microlenses for imaging fast dynamic processes or for high-throughput imaging:
- widefield mode for detection of weak signals and for light-sensitive samples
- transmitted light imaging - brightfield and differential phase contrast for transparent samples and samples naturally providing high contrast images.
Includes a fluorescence source in the form of 4 fixed excitation lines: 405 nm, 488 nm, 561 nm and 638 nm and a broad-spectrum LED source for transmitted light observation.
For working with photosensitive samples, the microscope is equipped with a sufficiently sensitive detector (QE 82%) allowing fast scanning.
The entire system is controlled by a powerful workstation equipped with Fusion acquisition software with image deconvolution capability and IMARIS analysis software for immediate 3D visualization of acquired data.
